Hiroaki Nitta

7851145, Dec 14, 2010

Dec 27, 2006

12/096075

Hiroaki Nitta - Oro Valley AZ, US
Thomas M. Grogan - Tucson AZ, US
Phillip Miller - Tucson AZ, US

Ventana Medical Systems, Inc. - Tucson AZ

C12Q 1/00

435 4, 435 723

The present disclosure provides methods of diagnosing cervical dysplasia and cervical cancer by detecting Na, K-ATPase expression. For example, it is demonstrated herein that expression of the Na, K-ATPase β1-subunit increases in cervical dysplasia and cervical cancer, relative to normal cervical tissue. Also provided are methods of treating cervical dysplasia and cervical cancer by decreasing the biological activity of the Na, K-ATPase. For example, therapeutically effective amounts of one or more Na, K-ATPase inhibitors can be applied to the cervix to treat cervical dysplasia and cervical cancer.

8481270, Jul 9, 2013

Aug 21, 2009

13/059274

Richard Gniewek - Oro Valley AZ, US
Michael Farrell - Tucson AZ, US
Hiroaki Nitta - Oro Valley AZ, US
Megan Lehrkamp - Castro Valley CA, US
Jerome Kosmeder - Tucson AZ, US
Brian Daniel Kelly - Tucson AZ, US
Thomas Grogan - Tucson AZ, US
Fabien Gaire - Oro Valley AZ, US
Mary Padilla - Tucson AZ, US
Christopher Bieniarz - Tucson AZ, US

Ventana Medical Systems, Inc. - Tucson AZ

C12Q 1/68
G01N 33/58
G01N 33/53

435 619, 435 61, 435 611, 435 617, 435 71, 435 79

The present invention provides a method and kit for detection of two or more target molecules in a single tissue sample, such as for gene and protein dual detection in a single tissue sample. Methods comprise treating a tissue sample with a first binding moiety that specifically binds a first target molecule. Methods further comprise treating the tissue sample with a solution containing a soluble electron-rich aromatic compound prior to or concomitantly with contacting the tissue sample with a hapten-labeled binding moiety and detecting a second target molecule. In one example, the first target molecule is a protein and the second is a nucleic acid sequence, the first target molecule being detected by immunohistochemistry and the second by in situ hybridization. The disclosed method reduces background due to non-specific binding of the hapten-labeled specific binding moiety to an insoluble electron rich compound deposited near the first target molecule.

20030044823, Mar 6, 2003

Apr 30, 2002

10/136714

Catherine Wolf - Eckbolsheim, FR
Hiroaki Nitta - Oro Valley AZ, US
Thomas Grogan - Tucson AZ, US
Jacques Cavadenti - Strasbourg, FR
Anthony Hartman - Tucson AZ, US
Angela Sattler - Tucson AZ, US
Jennifer Wong - Tucson AZ, US

C12Q001/68
C12N001/08

435/006000, 435/270000

The invention provides novel reagents, reagent kits, and methods for automated hybridization. More particularly, the invention provides reagents, reagent kits, and methods for automated in situ hybridization and automated hybridization on a microarray. The use of automated instruments for in situ hybridization and microarray hybridization dramatically reduces the amount of labor and time involved and also facilitates standardization of protocols and consistency between results.

20080299555, Dec 4, 2008

May 30, 2007

11/809024

Hiroaki Nitta - Oro Valley AZ, US
Thomas M. Grogan - Tucson AZ, US

C12Q 1/68
C12Q 1/00
C12Q 1/26
C12Q 1/28
G01N 33/53

435 6, 435 4, 435 25, 435 28, 435 79

The present invention provides compositions, kits, assembles of articles and methodology for detecting multiple target molecules in a sample, such as in a tissue sample. In particular, site-specific deposition of elemental metal is used in conjunction with other means of detection, such as other chromogenic, radioactive, chemiluminescent and fluorescent labeling, to simultaneously detect multiple targets, such a gene, a protein, and a chromosome, in a biological sample. More particularly the multiple targets may be labeled with the specifically deposited metal and other chromogenic labels to allow chromogenic immunohistochemical (IHC) detection in situ by using bright field light microscope.