Inventors:
Hyesook Kim - Bloomfield Hills MI, US
Alan A. Dombkowski - Canton MI, US
Raymond F. Novak - Orchard Lake MI, US
Brian B. Haab - Jenison MI, US
Assignee:
DETROIT R&D, INC. - Bloomfields Hills MI
International Classification:
C40B 30/04
C40B 40/10
C40B 50/06
Abstract:
An antibody microarray screen including a substrate, monoclonal and polyclonal antibodies that are purified immunoglobins, wherein the antibodies are spotted on predetermined positions on the substrate, and fluids unprocessed for immunoglobulin isolation (e.g., anti-sera, ascites fluids, or hybridoma culture media), wherein the unprocessed fluids are spotted on the predetermined positions on the substrate. Production of drug-metabolizing enzyme antibody microarrays containing closely related cytochromes P450 is disclosed. Methods of manufacturing an antibody microarray, an internal control molecule for use in an antibody microarray, a method of determining optimal spotting concentrations of IgG and a method to increase a detectable signal with microarray analysis are disclosed.