Barbara J England

55893937, Dec 31, 1996

Apr 24, 1995

8/427508

Michael D. Fiechtner - Highland Park IL
John M. Ramp - Gurnee IL
Barbara J. England - Milwaukee WI
Mary J. Annino - Arlington Heights IL

Abbott Laboratories - Abbott Park IL

G01N 3396

436 15

The invention is a rapid, continuous test for glycated hemoglobin using a non-equilibrium affinity binding method. Agarose beads derivatized with 3-aminophenylboronic acid specifically bind glycated hemoglobin. This solid phase is incorporated into a sample processor card, modified to mix and to separate the test solution from the solid phase prior to absorbance readings. Two absorbance readings are made on the test solution, one immediately after mixing the reagent/diluent with the specimen, and one after a significant amount of binding has occurred. A linear correlation between total glycated hemoglobin and hemoglobin A. sub. 1c permits standardization and reporting of units equivalent to % hemoglobin A. sub. 1c. Stable glycated hemoglobin solutions for use as standards in the assay, and a method for preparing the standards are also disclosed.

56863160, Nov 11, 1997

Apr 28, 1995

8/431398

Michael D. Fiechtner - Highland Park IL
John M. Ramp - Gurnee IL
Barbara J. England - Milwaukee WI
Mary J. Annino - Arlington Heights IL

Abbott Laboratories - Abbott Park IL

G01N 33543
G01N 33545
G01N 33537
G01N 33538

436518

The invention is a rapid, continuous test for glycated hemoglobin using a non-equilibrium affinity binding method. Agarose beads derivatized with 3-aminophenylboronic acid specifically bind glycated hemoglobin. This solid phase is incorporated into a sample processor card, modified to mix and to separate the test solution from the solid phase prior to absorbance readings. Two absorbance readings are made on the test solution, one immediately after mixing the reagent/diluent with the specimen, and one after a significant amount of binding has occurred. A linear correlation between total glycated hemoglobin and hemoglobin A. sub. 1c permits standardization and reporting of units equivalent to % hemoglobin A. sub. 1c. Stable glycated hemoglobin solutions for use as standards in the assay, and a method for preparing the standards are also disclosed.

54016398, Mar 28, 1995

Aug 25, 1992

7/935677

Louis Saldivar - Kenosha WI
Barbara J. England - Milwaukee WI

Abbott Laboratories - Abbott Park IL

C12Q 154
G01N 3300

435 14

A stabilized anoxic diagnostic reagent solution comprising an oxygen labile reagent, glucose oxidase, glucose, a hydrogen donor, sterile membrane fragments derived from bacteria having membranes containing an oxygen transfer system, and a reagent binding agent. The stabilized reagent has at least six months storage stability at 2. degree. -8. degree. C. and open vial stability of at least three weeks.