Inventors:
Ming Luo - Birmingham AL
Bingdong Sha - Birmingham AL
Assignee:
University of Alabama Research Foundation - Birmingham AL
International Classification:
C12N 700
C12N 704
C07K 1411
C12Q 170
Abstract:
The matrix protein, M1, of influenza virus strain A/PR/8/34 has been purified from virions and crystallized. The crystals consist of a stable fragment (18 Kd) of the M1 protein. X-ray diffraction studies indicated that the crystals have a space group of P3. sub. t 21 or P3. sub. 2 21. Vm calculations showed that there are two monomers in an asymmetric unit. A crystallized N-terminal domain of M1, wherein the N-terminal domain of M1 is crystallized such that the three dimensional structure of the crystallized N-terminal domain of M1 can be determined to a resolution of about 2. 1. ANG. or better, and wherein the three dimensional structure of the uncrystallized N-terminal domain of M1 cannot be determined to a resolution of about 2. 1. ANG. or better. A method of purifying M1 and a method of crystallizing M1.