Inventors:
Don B. Clewell - Ann Arbor MI
Mary C. Gawron-Burke - Ann Arbor MI
Assignee:
Board of Regents of The University of Michigan - Ann Arbor MI
International Classification:
C12P 2100
C12N 100
C12N 1500
Abstract:
Processes and new genetic materials are provided for cloning specific DNA fragments by using a unique conjugative transposon designated Tn916. The transposon is used to first target specific genes by insertional inactivation. A restriction fragment containing the inserted transposon is then inserted into a plasmid vector and transformed into Escherichia coli or other suitable host by selection for the transposon encoded tetracycline (Tc) resistance. The transformants so produced are then grown in the absence of tetracycline conditions under which Tn916 excises from the chimeric plasmid thus restoring the integrity of the DNA into which the transposon was originally inserted. This process provides a new and useful way of producing new life forms that are useful for making desired products having established utility.
