Kailash C. Chadha - West Seneca NY
Eugene Sulkowski - Buffalo NY
Health Research (Roswell Park Division) - Buffalo NY
A process for the production and purification of human interferon derived from leukocyte of lymphoblastoid cell origin characterized by a sequence of steps involving chromatography of a solution of crude interferon on porous glass, utilizing three new classes of eluants for recovering the interferon bound to the porous glass, followed by cation exchange chromatography and finally chromatography on a hydrophobic sorbent material which process produces a final purified interferon in its natural form containing those protein components which are normally labile to low pH treatment along with those components which are pH stable. In one aspect, the improved method of purification also includes the optional molecular filtration of the crude interferon prior to initiating the purification process. In yet another embodiment, a new process for producing
The invention described herein was made, in part, in the course of work under grant No. NOI-HB-2920 awarded by the National Heart, Lung And Blood Institute.