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Eugene Sulkowski Phones & Addresses

  • 443 Parkside Ave, Buffalo, NY 14216
  • Aiea, HI

Publications

Wikipedia

AmPol Eagle Citizen of the Year Award the free ...

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2010 Leonard Amborski; 2006 Arthur M. Michalek; 1981 Dr. Felix Milgrom; 1980 Dr. Francis Bajer; 1979 Eugene Sulkowski, Ph. D; 1978 Dr. Michael ...

Us Patents

Method For Detecting Psa And Its Molecular Forms Using Thiophilic Gel

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US Patent:
6379550, Apr 30, 2002
Filed:
Jul 24, 2000
Appl. No.:
09/624692
Inventors:
Kailash C. Chadha - Williamsville NY
Eugene Sulkowski - Buffalo NY
Elzbieta Kawinski - Orchard Park NY
Assignee:
Health Research, Inc. - Buffalo NY
International Classification:
B01D 1508
US Classification:
210635, 210656, 2101982, 435 723, 435 74
Abstract:
A method for capturing PSA and its molecular forms that may be in a fluid biological material including the steps of: preparing a chromatographic column by placing a thiophilic gel in a column where the thiophilic gel is formed from a water insoluble polymer where the surface of the gel is provided with thiophilic moieties that bind PSA in the presence of an adsorption liquid but that will release PSA upon elution with an eluting liquid, said thiophilic moieties comprising a two part structure wherein one part can be characterized as a hydrophilic electron acceptor and the other part is sulfur which acts as an electron donor; selecting a sample of a fluid biological material to be tested for PSA and its complexes; introducing the sample into the column; eluting the sample through the column; rinsing the column with adsorption liquid to remove materials that are unbound to the thiophilic gel; and capturing PSA and its complexes in eluted column fractions.

Method For Detecting Psa And Its Molecular Forms Using Thiophilic Gel On Magnetic Beads

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US Patent:
6692643, Feb 17, 2004
Filed:
Apr 29, 2002
Appl. No.:
10/134235
Inventors:
Eugene Sulkowski - Buffalo NY
Kailash C. Chadha - Williamsville NY
Elzbieta Kawinski - Orchard Park NY
Assignee:
Health Research, Inc. - Buffalo NY
International Classification:
B01D 1508
US Classification:
210635, 210656, 2101982, 210222, 435 723, 435 74
Abstract:
A method for capturing PSA and its molecular forms that may be in a fluid biological material including the steps of: preparing a bed of magnetic beads by binding thiophilic ligands to the beds where the thiophilic ligands bind PSA and its complexes, said thiophilic ligands comprising a two part structure wherein one part can be characterized as a hy6drophilic electron acceptor and the other part is sulfur which acts as an electron donor; selecting a sample of a fluid biological material to be tested for PSA and its complexes; introducing the sample into the magnetic beads bound to thiophilic ligands so that PSA and its complexes bind to the thiophilic ligand; and magnetically removing the beads from unbound portions of the sample.

Method For The Production And Purification Of Human Leukocyte Interferon

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US Patent:
44850387, Nov 27, 1984
Filed:
Oct 19, 1982
Appl. No.:
6/435295
Inventors:
Kailash C. Chadha - West Seneca NY
Eugene Sulkowski - Buffalo NY
Assignee:
Health Research (Roswell Park Division) - Buffalo NY
International Classification:
C07C10352
A61K 4502
C12P 2100
C07G 700
US Classification:
260112K
Abstract:
A process for the production and purification of human interferon derived from leukocyte of lymphoblastoid cell origin characterized by a sequence of steps involving chromatography of a solution of crude interferon on porous glass, utilizing three new classes of eluants for recovering the interferon bound to the porous glass, followed by cation exchange chromatography and finally chromatography on a hydrophobic sorbent material which process produces a final purified interferon in its natural form containing those protein components which are normally labile to low pH treatment along with those components which are pH stable. In one aspect, the improved method of purification also includes the optional molecular filtration of the crude interferon prior to initiating the purification process. In yet another embodiment, a new process for producing The invention described herein was made, in part, in the course of work under grant No. NOI-HB-2920 awarded by the National Heart, Lung And Blood Institute.

Method For The Large Scale Purification Of Human Fibroblast Interferon

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US Patent:
46580174, Apr 14, 1987
Filed:
Feb 14, 1984
Appl. No.:
6/579994
Inventors:
Wlodzimierz E. Dembinski - Buffalo NY
Eugene Sulkowski - Buffalo NY
Assignee:
Health Research, Inc. (Roswell Park Division) - Buffalo NY
International Classification:
C07K 320
C07K 1526
A61K 4502
US Classification:
530351
Abstract:
A process for the large scale purification of human fibroblast interferon produced by human diploid fibroblasts under superinduction conditions characterized by a sequence of four chromatographic steps namely: (1) separation on CM-agarose; (2) separation on ConA-agarose; (3) separation on phenyl-agarose; and (4) elimination of ethylene glycol using CM-agarose which process yields a final overall recovery not less than 60% of the crude interferon starting material and having a specific activity not less than 1. times. 10. sup. 8 units per mg of protein.
Eugene A Sulkowski from Buffalo, NYDeceased Get Report