Inventors:
Zoltan Timar - Loveland CO, US
Gergo Hegedus - Loveland CO, US
Assignee:
Agilent Technologies, Inc. - Santa Clara CA
International Classification:
H01J 49/26
G01N 21/00
G06F 19/00
US Classification:
436 94, 436 93, 436 91, 422 8205, 422 681, 422 50, 702 19, 702 1
Abstract:
A computer-implemented method for confirming the nucleotide sequence of an oligonucleotide is provided. In certain embodiments, the method comprises: a) inputting the nucleotide sequence of an oligonucleotide; b) executing an algorithm that provides the predicted molecular formulas of fragments of the oligonucleotide; c) comparing the predicted m/z values of the predicted molecular formulas to experimentally-obtained m/z values obtained by analysis of the oligonucleotide by tandem mass spectrometry to determine if the predicted masses correspond with the experimentally-obtained masses. The method may be used, for example, to confirm the identity of an oligonucleotide after it is synthesized.