Inventors:
Robert Dirksen - Pittsford NY, US
Heping Cheng - Baltimore MD, US
Shey-Shing Sheu - Pittsford NY, US
Wang Wang - Ann Arbor MI, US
Linda Groom - Lyons NY, US
International Classification:
C12Q 1/02
C07K 14/00
C12N 15/11
C12N 5/06
A01K 67/027
US Classification:
435 29, 530350, 536 231, 435325, 800 13
Abstract:
Protein probes and methods for measuring real-time changes in intracellular superoxide formation are provided. The probes include superoxide sensitive variants of yellow fluorescent and green fluorescent proteins. The probes, or nucleic acids encoding the probes, may be delivered to cells or organisms. Changes in the fluorescence of the probes may then be detected using standard real-time fluoroscopy techniques.