Inventors:
Janice A. Kolberg - Hercules CA
Michael S. Urdea - Alamo CA
Assignee:
Bayer Corporation - East Walpole MA
International Classification:
C12Q 170
C12Q 168
C12P 1934
Abstract:
Synthetic oligonucleotides which are useful as probes in a solution phase sandwich hybridization assay for the detection of HTLV-1 are described. The oligonucleotides have a first segment comprising a nucleotide sequence substantially complementary to a segment of HTLV-1 nucleic acid, and a second segment comprising a nucleotide sequence which is not complementary to HTLV-1 nucleic acid but is substantially complementary to an oligonucleotide unit of a nucleic acid multimer or to an oligonucleotide bound to a solid phase. Amplified nucleic acid hybridization assays using the probes are exemplified.