US Patent:
20120135425, May 31, 2012
Inventors:
Jeffrey Lakritz - Columbus OH, US
Grigori Andreevich Bannikov - Dublin OH, US
Tatiana Karelina - Dublin OH, US
Assignee:
THE OHIO STATE UNIVERSITY RESEARCH FOUNDATION - Columbus OH
International Classification:
G01N 33/573
G01N 21/64
Abstract:
A method for detecting a haptoglobin-matrix metalloproteinase 9 (Hp-MMP 9) complex in a biological sample. The sample includes incubating the biological sample with a capture reagent immobilized on a solid support to bind Hp-MMP 9 to the capture reagent. The capture reagent includes a monoclonal antibody that binds MMP9. The method detects Hp-MMP 9 bound to the immobilized capture reagent by contacting the bound Hp-MMP 9 with a detectable antibody that binds to Hp.