John A Maples

6913932, Jul 5, 2005

Aug 23, 2002

10/226825

John A. Maples - Miami FL, US
Lori A. Charie - Pembroke Pines FL, US
Daniel James Flagler - Miami FL, US
Rhonda Ann Mills - Pembroke Pines FL, US
Richard Timmons - Miami FL, US

Beckman Coulter, Inc. - Fullerton CA

G01N033/00
G01N001/00

436176, 436 8, 436 18, 436111, 436128, 436130, 436 63, 436 69, 422 61, 2524081

A stabilization reagent composition, particularly desirable for stabilizing blood samples containing platelets, contains reactants that generate multiple species of formaldehyde-ammonium complexes; at least one inhibitor of phosphatase enzymatic activity; and at least one inhibitor of protease enzymatic activity. Blood samples and other tissues are stabilized by this composition. Such stabilized samples are produced by methods for stabilizing the tissue by contacting the sample with the composition.

6955872, Oct 18, 2005

Mar 20, 2003

10/392518

John A. Maples - Miami FL, US
Lidice L. Lopez - Miami FL, US
Nancy Torke - Miami FL, US

Coulter International Corp. - Miami FL

C12Q001/68

435 4, 435 6

A composition for enhancing differential staining of RNA, DNA and granules in a sample comprising cells contains a first fluorescent dye that can bind specific binding sites and non-specific binding sites in the sample. This first dye emits fluorescence at a first wavelength. The composition contains at least an additional component, which is a second non-intercalating dye in the composition that competes with said first dye for binding to the nonspecific binding sites, or a permeabilizing agent to enhance permabilization of the dyes into the cells, or both. The molar ratio of the second dye and the first dye is at least about 20:1.

7300797, Nov 27, 2007

Sep 14, 2001

09/951903

Andre van Agthoven - Marseilles, FR
Jean-Pierre Daziano - Marseilles, FR
John Allen Maples - Miami FL, US

Immunotech, S.A. - Marseilles

G01N 31/00

436 17, 356 73, 356317, 356336, 356 39, 516 77

A multifunctional reagent for erythrocytes containing an amount sufficient to produce the lysis of erythrocytes or the sphering of erythrocytes in such a way that they can be detected by a cytometer or an automatic counting device, of a carbamate or of an agent inducing the formation by the erythrocytes, from carbonate and from a nitrogenated heterocycle or ammonium ions, of a carbamate combined with the absorption of COby said erythrocytes, process for lysing or sphering erythrocytes and preparation process for leucocytes.

7354773, Apr 8, 2008

May 14, 2003

10/437695

Oilda Rubio - Miami FL, US
Carlos Aparicio - Miami FL, US
John A. Maples - Miami FL, US
Julie Wilkinson - Fort Lauderdale FL, US
Cecilia Smith - Miami FL, US
Frank J. Lucas - Boca Raton FL, US

Beckman Coulter, Inc. - Fullerton CA

G01N 1/18

436177, 435 2, 435 725, 435 405, 435366, 435372, 435398, 435400, 435401, 4352872, 435971, 436521, 436522, 436536, 436538, 436546, 436 17, 436 45, 436 47

A method and apparatus for preparing biological cell samples for intracellular analysis. The invention is based upon the recognition that many of the steps of the conventional methods for such sample preparation can be eliminated, leading to a process that readily lends itself to automation and the advantages associated therewith. The method of the invention comprises the steps of (a) cell-fixation, (b) permeabilization and (c) staining (or labeling) of intracellular molecules of interest by probes that are readily detectable by flow cytometric techniques, all without any intervening cell-washing (and re-suspension) steps. Rather, the single cell-washing step is effected after these three steps have been carried out. Preferably, the washing step is carried out by passing the fixed, permeabilized and stained cell sample through a semi-permeable membrane that serves to filter out (by transmission) interferants to waste while retaining the cells of interest.

7678583, Mar 16, 2010

Oct 8, 2007

11/868580

Andre van Agthoven - Marseilles, FR
Jean-Pierre Daziano - Marseilles, FR
John Allen Maples - Miami FL, US

Immunotech, S.A. - Marseilles

G01N 33/555

436522, 424520, 435 2, 435 724, 435269

A multifunctional reagent for erythrocytes containing an amount sufficient to produce the lysis of erythrocytes or the sphering of erythrocytes in such a way that they can be detected by a cytometer or an automatic counting device, of a carbamate or of an agent inducing the formation by the erythrocytes, from carbonate and from a nitrogenated heterocycle or ammonium ions, of a carbamate combined with the absorption of COby the erythrocytes, process for lysing or sphering erythrocytes and preparation process for leucocytes.

20050069958, Mar 31, 2005

Sep 26, 2003

10/672477

Rhonda Mills - Pembroke Pines FL, US
Jorge Quintana - Tavernier FL, US
John Maples - Miami FL, US
Paul Scibelli - Miami FL, US

G01N033/53
G01N033/567

435007200

A method for evaluating a biological sample containing at least one cell type bearing at least one cellular target and at least one soluble analyte, involves adding to a single container the sample with (i) at least one soluble ligand that binds the cellular target, (ii) either at least one soluble ligand that binds the soluble analyte or at least one competing soluble analyte; and (iii) a solid phase capture medium that binds directly to the soluble analyte, indirectly to the soluble analyte, or to the soluble ligand that binds to the soluble analyte. Various complexes involving the capture medium and soluble analyte, the capture medium and the soluble ligand, and/or the cellular target and a soluble ligand are simultaneously analyzed without physically separating the complexes.

20060024744, Feb 2, 2006

Jul 28, 2004

10/515073

Rhonda Mills - Pembroke Pines FL, US
Jorge Quintana - Tavernier FL, US
John Maples - Miami FL, US
Paul Scibelli - Miami FL, US
Wataru Hashimoto - Kanagawa, JP

G01N 33/53
G01N 33/567

435007100, 435007200

Methods are provided for monitoring treatment of a subject using heparin therapy or a ligand, such as an CD20 or CD52 antibody, that binds to a cellular marker and to which the subject may develop masking reactions or auto-antibodies that inhibit evaluation of treatment. The methods involve adding to a single container a sample containing cells that express the cellular target with (i) a soluble ligand that binds the cellular target, (ii) a soluble ligand that binds the soluble analyte or a competing soluble analyte; and (iii) a capture medium that binds directly to the soluble analyte, indirectly to the soluble analyte, or to the soluble ligand that binds to the soluble analyte. Complexes formed in the container by interaction of these components are substantially simultaneously analyzed and quantitated without physically separating the complexes. Kits for performing the assays are also provided.

20070015134, Jan 18, 2007

Jul 15, 2005

11/183132

John Maples - Miami FL, US
Rhonda Mills - Pembroke Pines FL, US
Daniel Flagler - Miami FL, US
Cecilia Smith - Lake Placid FL, US

Beckman Coulter, Inc. - Fullerton CA

C12Q 1/00
G01N 33/567
G01N 33/53

435004000, 435007200, 977900000

The invention relates to quantitative stabilized cell reference control products, their methods of manufacture, and their methods of use. Receptors, antigens, and ligands associated with stabilized cells may be quantitated by the methods of the present invention. Those quantitated stabilized cells may then be used to determine the receptor, antigen, and/or ligand density of unknown samples.