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Jonathan J Mitschelen

from Edmond, OK
Age ~76
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Also known as:
Johathan Mitschelen, Jonathan J Mitschele
Phone and address:
7500 E Sorghum Mill Rd, Edmond, OK 73034
Related to:
Denise Jones Ellis, 72Lefenton Ellis, 74Krista K Bracy, 44Aaron J Mitschelen, 44Matthew C Mitschelen, 41Linda G Mitschelen, 75
Connected to:
David C Mitschelen, 54Mark A Mitschele, 69Fred Mitschke, 83Brian P Mitsdarffer, 49Edmund F Mitsdarfer, 95Jodi K Mitsiopoulos, 56Georgia Mitsis, 85Shaun Mitsky, 41Kathryn A Mitson, 47Joanne D Mitsos, 53

Jonathan Mitschelen Phones & Addresses

  • 7500 E Sorghum Mill Rd, Edmond, OK 73034
  • Oklahoma City, OK
  • 29 Straub Dr, Pleasant Valley, NY 12569 (845) 635-1336
  • Hopewell Junction, NY
  • Perkiomenville, PA
  • Fishkill, NY
  • Fairfax, VA
  • 7500 E Sorghum Mill Rd, Edmond, OK 73034 (845) 635-1336

Work

Position: Service Occupations

Resumes

Resumes

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Jonathan Mitschelen

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Publications

Us Patents

Methods For Purification Of Recombinantly Produced Proteins

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US Patent:
55301002, Jun 25, 1996
Filed:
May 7, 1990
Appl. No.:
7/519709
Inventors:
Thomas L. J. Darling - Lafayette Hill PA
Lida Y. Akhnana - Audubon PA
Jonathan J. Mitschelen - Perkiomenville PA
Michael E. Hrinda - Gwynedd Valley PA
Assignee:
Rhone-Poulenc Rorer Pharmaceuticals Inc. - Collegeville PA
International Classification:
C07K 308
C07K 312
C07K 320
C07K 1506
US Classification:
530383
Abstract:
Recovery of the 52/48 kDa tryptic fragment of vWF or peptide subfragments thereof produced in the form of inclusion bodies from recombinant host cells is carried out by providing a washed recombinant host cell suspension, adding a detergent and subjecting the cells to mechanical disruption. A second detergent is added, followed by another mechanical disruption and centrifugation to a pellet. The pellet is resuspended in a buffer and subjected to another mechanical disruption. Inclusion bodies are washed, resuspended and then recovered. In addition, endotoxins and DNA are removed from inclusion bodies containing the 52/48 kDa tryptic fragment of vWF or peptide subfragments thereof by mechanically disrupting the inclusion bodies in an aqueous buffer containing a detergent. A washed pellet is formed from these mechanically disrupted inclusion bodies and dissolved in a denaturant. Alkylation is then performed and the suspension is subjected to column chromatography, where the alkylated fragment or subfragment is recovered by elution.

Production And Purification Of Retroviral Particles Using Tentacle Anion Exchange

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US Patent:
56610238, Aug 26, 1997
Filed:
Mar 11, 1996
Appl. No.:
8/613920
Inventors:
Michael E. Hrinda - Gwynedd Valley PA
Christopher P. Prior - Wayne PA
Jonathan J. Mitschelen - Perkiomenville PA
Thomas W. Irish - Pottstown PA
David M. Weber - Phoenixville PA
Richard S. Gore - Southampton PA
James J. Harter - Media PA
Pierre M. Bay - Philadelphia PA
George C. Tarr - Norristown PA
Assignee:
The Immune Response Corporation - Carlsbad CA
International Classification:
C12N 702
US Classification:
435239
Abstract:
This invention is directed to a continuous process for producing viral particles by providing in a perfused growth medium a population of viable virally infected non-lytic cells, and removing medium containing said cells at a rate to maintain the steady-state log-phase growth of cells remaining in the perfused growth medium. The invention is also directed to a process for purifying retroviral particles by passing a solution comprising the retroviral particles and contaminants through an anion exchange resin, and eluting the retroviral particles from the resin. The preferred exchange resin is a tentacle anion exchange resin.
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Jonathan J Mitschelen from Edmond, OK, age ~76 Get Report