Inventors:
Margareta K. Tuckman - Tenafly NJ
Marcia S. Osburne - Lexington MA
Assignee:
American Cyanamid Company - Wayne NJ
International Classification:
C12Q 110
C12N 100
Abstract:
The present invention relates to a method for identifying a TonB inhibitor in a test sample comprising: (a) growing a TonB. sup. + microorganism in the presence of the test sample and a lethal agent, the activity of which is mediated by TonB; (b) identifying as positive a test sample with which the lethal activity of the agent is not observed; (c) growing on a low-iron medium a TonB. sup. + microorganism in the presence of the test sample identified as positive in (b); (d) confirming as positive a test sample with which growth inhibition of the microorganism is observed on a low-iron medium.