Inventors:
Ramamoorthy Belagaje - Indianapolis IN
Janet K. Epp - Indianapolis IN
JoAnn Hoskins - Indianapolis IN
Hansen M. Hsiung - Indianapolis IN
George L. Long - Indianapolis IN
Brigitte E. Schoner - Zionsville IN
Assignee:
Eli Lilly and Company - Indianapolis IN
International Classification:
C12P 2100
C12P 2102
C12N 1500
C12N 120
C12N 700
C07H 1512
Abstract:
The present invention comprises improved derivatives of the tryptophan operon useful for expressing fused gene products in E. coli and relate organism. Two of the improved derivatives disclosed are encoded on 0. 43 and 0. 55 kb EcoRI restriction fragments from plasmids pCZ20 and pLEBGH2 (in strains NRRL B-15881 and NRRL B-15882), respectively. The modified derivatives have been placed on recombinant DNA cloning and expression vectors. A variety of expression vectors have been constructed that drive expression of fused gene products. Two novel gene sequences, encoding insulin-like growth factors I and II, have been expressed with the modified tryptophan operon system.