Yoshitsugi H Hokama

6770490, Aug 3, 2004

Jul 15, 1998

09/115797

Yoshitsugi Hokama - Honolulu HI
Joanne S. M. Ebesu - Honolulu HI
Warren E. Takenaka - Honolulu HI
Robert E. Bourke - Honolulu HI
Patrick K. Sullivan - Honolulu HI

Oceanit Test Systems, Inc. - Honolulu HI

G01N 3353

436808, 435 71, 435 72, 435 405, 436164, 436168, 436177, 436538, 436541, 436805, 436807, 436823, 436824, 436 20

An immunological test kit suitable for the rapid detection of ciguatoxin and related low molecular weight lipid polyether marine toxins in fish tissues in the field, home or laboratory. The test procedure utilizes a synthetic membrane laminated onto one end of a solid plastic stick, which is immersed into alcohol with a piece of fish tissue. The membrane is then removed and dried thoroughly, then placed in the suspension of mixed colored beads coated with anti-ciguatoxin. The membrane is then rinsed in water and the intensity of the color compared with a standard range of colors formed (0-3+). The color intensity is proportional to the concentration of toxin in the piece of tissue. The kit includes the synthetic membrane on a plastic stick, a suspension of mixed colored beads coated with anti-ciguatoxin antibody, testing instruments, and a supply of solvent.

48163929, Mar 28, 1989

Jun 1, 1987

7/056130

Yoshitsugi Hokama - Honolulu HI

Research Corporation of the University of Hawaii - Honolulu HI

G01N 33543

435 7

A rapid stick test for the detection of ciguatoxin and other polyether toxins from fish tissue is disclosed. This is a relatively simple and rapid field test utilizing enzyme immunoassay technology for the detection of lipid toxin which can be assessed in ten minutes or less in the field. The invention is both a procedure and a kit for conducting the test. The kit includes a bamboo sampling stick, buffer reagent, anti-ciguatoxin horseradish peroxidase conjugate, substrate reagent, fixation reagent, blotter, filter paper, and medicine dropper.