US Patent:
20230078644, Mar 16, 2023
Inventors:
- Elkridge MD, US
Robert Altavela - Webster NY, US
Michael Carlotta - Lilburn GA, US
David Cigna - Rochester NY, US
John C. Detter - Melbourne Beach FL, US
Steven Dietl - Ontario NY, US
Charles Facchini - Webster NY, US
Todd Haran - Bloomfield NY, US
Roger Markham - Webster NY, US
Michael Murray - Bloomfield NY, US
Scott Rosebrough - Avon NY, US
Jeffrey Serbicki - Holley NY, US
Qing Yang - Pittsford NY, US
International Classification:
B01L 3/00
C12Q 1/6806
B01L 7/00
Abstract:
A device for detecting nucleic acids in a biological sample has a sample port, a lysis station and a sample conduit configured to mix a sample and lysis agent to form a sample-lysis mixture, pass the sample-lysis mixture across a solid-state membrane to capture nucleic acids in the biological sample therein, and receive the remainder of the sample-lysis mixture in a waste chamber. The wash station is configured to introduce the wash solution following the sample-lysis mixture, pass the wash solution across the solid-state membrane to purify nucleic acids captured therein, and receive the wash solution from the solid-state membrane in the waste chamber. The elution station is configured to pass the eluent across the solid-state membrane, elute captured nucleic acids from the solid-state membrane, and pass the captured nucleic acids into one or more reaction chambers for amplifying and detecting the captured nucleic acids.
