Rick W Ye

6429003, Aug 6, 2002

Feb 15, 2000

09/504357

Laura Anne Bedzyk - Odessa DE
Rick Weizhang Ye - Hockessin DE

E. I. du Pont de Nemours and Company - Wilmington DE

C12N 121

4352523, 435191, 43525411, 4353201, 43525231, 4352542, 43525233, 536 231, 536 232, 536 237

This invention relates to the isolation of nucleic acid fragments from Pseudomonas sp. strain G-179 that encode periplasmic nitrate reductase and nitric oxide reductase enzymes. The enzymes are useful in denitrification reactions and for the identification of other denitrifying bacteria. In addition, this invention also relates to the construction of chimeric genes encoding all or a substantial portion of a bacterial nitric oxide reductase or a bacterial periplasmic nitrate reductase enzymes, in sense or antisense orientation, wherein the expression of the chimeric genes results in production of altered levels of the nitric oxide reductase or periplasmic nitrate reductase in a transformed host cell.

6537786, Mar 25, 2003

Aug 22, 2001

09/934899

Mattheos Koffas - Wilmington DE
James M. Odom - Kennett Square PA
Siqun Wang - Wilmington DE
Tao Wang - Hockessin DE
Rick W. Ye - Hockessin DE

E. I. du Pont de Nemours and Company - Wilmington DE

C12P 1904

435101, 435 41, 435 72, 435183, 435193, 4352523, 4353201, 536 232

Genes have been isolated from a Methylomonas sp encoding elements of the exopolysaccharide biosynthetic pathway. The genes and gene products are the first isolated from an organisms capable of utilizing single carbon (C1) substrates as energy sources. The genes are useful for engineering other C1 utilizing microorganisms to make altered levels of exopolysaccharide which is used in a variety of commercial applications.

6555353, Apr 29, 2003

Aug 22, 2001

09/934901

Mattheos Koffas - Wilmington DE
Kelley C. Norton - Avondale PA
James M. Odom - Kennett Square PA
Rick W. Ye - Hockessin DE

E. I. du Pont de Nemours and Company - Wilmington DE

C12N 912

435194, 4352523, 43525231, 43525233, 43525234, 43525235, 43525411, 4352542, 43525421, 43525422, 43525423, 4352543, 4353201, 536 232

Genes have been isolated from a Methylomonas sp encoding enzymes in the carbon flux pathway. The genes encode a 2-keto-3-deoxy-6-phosphogluconate (KDPGA) and a fructose bisphosphate aldolase (FFBPA), as well as numerous other genes. The genes will be useful in C1 metabolizing microorganisms for the manipulation of the carbon flux pathway.

6617148, Sep 9, 2003

Jun 26, 2001

09/891641

Laura A. Bedzyk - Odessa DE
Tao Wang - Hockessin DE
Rick Ye - Hockessin DE

E. I. du Pont de Nemours and Company - Wilmington DE

C12N 120

43525231, 435 691, 435243, 4352523, 4353201, 4352525, 435832, 435833, 536 232

Genes have been identified in the Bacillus genome that are responsive to various metabolic conditions and growth cycle changes. The new responsiveness of these genes allows for their use in regulated gene expression in Bacillus sp. and for the monitoring of bioreactor health.

6767744, Jul 27, 2004

Dec 17, 2002

10/321210

Mattheos Koffas - Wilmington DE
Kelley C. Norton - Avondale PA
James M. Odom - Kennett Square PA
Rick W. Ye - Wilmington DE

E. I. du Pont de Nemours and Company - Wilmington DE

C12N 121

435471, 435194, 4352523, 435440, 435476, 536 232

Genes have been isolated from a Methylomonas sp encoding enzymes in the carbon flux pathway. The genes encode a 2-keto-3-deoxy-6-phosphogluconate (KDPGA) and a fructose bisphosphate aldolase (FFBPA), as well as numerous other genes. The genes will be useful in C1 metabolizing microorganisms for the manipulation of the carbon flux pathway.

6773905, Aug 10, 2004

Dec 17, 2002

10/320874

Mattheos Koffas - Wilmington DE
Kelley C. Norton - Avondale PA
James M. Odom - Kennett Square PA
Rick W. Ye - Wilmington DE

E. I. du Pont de Nemours and Company - Wilmington DE

C12N 912

435194

Genes have been isolated from a Methylomonas sp encoding enzymes in the carbon flux pathway. The genes encode a 2-keto-3-deoxy-6-phosphogluconate (KDPGA) and a fructose bisphosphate aldolase (FFBPA), as well as numerous other genes. The genes will be useful in C1 metabolizing microorganisms for the manipulation of the carbon flux pathway.

6908992, Jun 21, 2005

Dec 17, 2002

10/320924

Mattheos Koffas - Wilmington DE, US
Kelley C. Norton - Avondale PA, US
James M. Odom - Kennett Square PA, US
Rick W. Ye - Wilmington DE, US

E. I. du Pont de Nemours & Company - Wilmington DE

C07H021/04
C12N009/88
C12N001/21
C12N001/15
C12N015/52

536 232, 435232, 4352523, 43525231, 43525233, 43525234, 43525235, 43525411, 4352542, 43525421, 43525422, 43525423, 4352543, 4353201

Genes have been isolated from a sp encoding enzymes in the carbon flux pathway. The genes encode a 2-keto-3-deoxy-6-phosphogluconate (KDPGA) and a fructose bisphosphate aldolase (FFBPA), as well as numerous other genes. The genes will be useful in C1 metabolizing microorganisms for the manipulation of the carbon flux pathway.

7026464, Apr 11, 2006

Oct 20, 2003

10/689200

Deana J. Dicosimo - Rockland DE, US
Hao Ni - New Castle DE, US
Stephen K. Picataggio - Wilmington DE, US
John E. Seip - Alloway NJ, US
Rick W. Ye - Hockessin DE, US
Tao Wang - Hockessin DE, US

E. I. du Pont de Nemours and Company - Wilmington DE

C07H 21/04

536 231

Genes have been identified in the genome that are responsive to various metabolic and growth conditions. The identified responsiveness of these genes allows for the use of their promoters in regulated gene expression in C1 metabolizing bacteria. In particular, the hps promoter, which in its native state drives the expression of 3-hexulose-6-phosphate synthase (HPS), was found to be useful for directing expression of heterolgous coding regions (e. g. , crtZ) in the obligate methanotroph sp. 16a.